Tuipulotu’s absence will not only be felt in Wales’ front row, she is also a big miss in camp.
Saturday’s skipper Keira Bevan said her loss is felt “massively”.
“She is such a big part of our playing squad, I think we’re all really starting to miss her a bit now, so if she could hurry up that would be brilliant.”
Second row Natalia John added: “It’s massively gutting because she is so good for the environment, she’s such a good human.
“We’re constantly all talking to her and she’s in good spirits, we just can’t wait to have her back in camp.”
Wales take on the Wallaroos for the second time in a week, having beaten them 31-24 in last Friday’s warm-up at Rodney Parade.
Hooker Rosie Carr suffered a shoulder injury in that game which ruled her out of the trip to South Africa, but Cunningham has called up her twin sister Katie.
“She’s settling in really well and we’re pleased to have her,” Cunningham said.