Back in April, Trump threatened to bomb civilian infrastructure in Iran, including bridges and power plants.

UN human rights chief Volker Türk responded at the time by saying: “Under international law, deliberately attacking civilians and civilian infrastructure is a war crime.”

The 1949 Geneva Conventions on humanitarian conduct in war prohibit attacks on sites considered essential for civilians.

“I’ll save the energy targets for last, but ultimately we’ll hit energy targets,” Trump said in an interview on Special Report with Bret Baier that aired on Tuesday night.

He said US negotiators had conveyed to their Iranian counterparts on Tuesday evening that they “‘better make a deal, or you’re not going to have anything left'”.

The escalation in rhetoric comes after Trump said a 20% toll he had threatened to impose in the Strait of Hormuz would be replaced by “massive” trade and investment deals with Gulf states.

The announcement came hours before the US military carried out a seven-hour wave of strikes on Iran and resumed a blockade of its ports.

US Central Command (Centcom) said it hit “dozens” of Iranian military targets near the Strait of Hormuz, with the aim to “further degrade Iran’s ability to threaten commercial shipping and civilian crews”.

At least seven Iranian military personnel were killed in US strikes on a base in the southeastern city of Bampur, Iran’s army said on Wednesday.

Meanwhile, Tehran fired missiles and drones on US targets in Jordan, Kuwait and Bahrain, Iranian state-run television IRIB reported.

In the early hours of Wednesday, Kuwait’s military said it was intercepting Iranian attack drones, while Bahrain activated air raid sirens.

“Kuwaiti air defences are currently engaging hostile drone attacks following the nefarious Iranian aggression,” the Kuwaiti army said. Bahrain’s interior ministry urged citizens and residents to remain calm and head to the nearest safe place.

The US also said Iran had “intentionally targeted civilians” in the region by attacking seven commercial ships, which had resulted in “nearly a dozen civilian crew members killed, missing, or injured”, the commander of Centcom said in a statement late on Tuesday.

The United Arab Emirates (UAE) said on Monday night that Iranian cruise missiles had targeted two national tankers, killing an Indian crew member and wounding eight others, four seriously.

Iran’s Islamic Revolutionary Guard Corps (IRGC) later confirmed the strikes via a statement to Telegram, where it said two tankers had ignored warnings, turned off navigation systems and attempted to pass through a mined route.

It was unclear which other attacks the Centcom statement was referring to. The IRGC did not immediately comment.

Renewed strikes between the US and Iran triggered a sharp rise in oil prices as tanker traffic through the Strait has virtually stalled.

It underscores the strategic importance of the waterway – with Iran accusing the US of interfering in its management of Hormuz – but controlling it means Tehran can also threaten the global economy.

On Monday, Trump declared that the US was now the “guardian” of the Strait of Hormuz, and vowed to impose a 20% charge on all cargo shipped through the waterway to pay for protecting it.

Raising the stakes further, Trump said the US would also reimpose its naval blockade on Iran in a bid to further squeeze the country’s struggling economy.

In his latest post on Truth Social, Trump wrote: “I have decided to replace the 20% United States Reimbursement Fee with Trade and Investment Deals that the various Gulf States will be making into the United States.

“Those Investments will be MASSIVE but, at the same time, extraordinarily good for them, and their future.”

The US president provided no further details.

He also said the strait “is open to ALL Ship traffic except for Iran” and that “oil is flowing like never before, thanks to the awesome Power of the United States Military”.

Speaking later, after talks in Washington with the new Iraqi Prime Minister Ali al-Zaidi, Trump said: “I don’t like the concept of a fee, but at the same time, it’s not fair that we’re protecting this Strait for the entire world.”

He said he had changed his initial fee plan after receiving numerous calls from Gulf leaders.

In response to Trump’s announcement, Iran said it would remain in control of the Strait of Hormuz.

Iran’s Deputy Foreign Minister Kazem Gharibabadi said Trump’s blockade decision “has, in a way, dismantled” an earlier agreed truce deal, the AFP news agency reported.

Gharibabadi also told state television, as quoted by Reuters news agency: “If the US thinks that by tightening its measures against us, its military actions and its economic blockade, we will return to negotiations, it is making a mistake.”

The US first imposed a naval blockade of all Iranian ports in April to put pressure on Tehran. Roughly five weeks later, the US military said it had redirected 100 commercial vessels and disabled four under the blockade.

The US lifted the blockade in June as part of a deal – known as a memorandum of understanding – between the two countries that aimed to end the conflict, but a dispute over the strait has become a key point of contention.

Meanwhile, shipping data shows traffic through the strait has slowed to a two-month low. The benchmark Brent Crude oil price has also risen sharply.

Additional reporting by Ella Kipling