On Tuesday, Trump threatened to target Iran’s energy infrastructure if Tehran failed to return to talks.
Iran’s top negotiator, Mohammad Bagher Ghalibaf, told state media Tehran had “no reason” to abide by any agreement that did not benefit the country.
He added that Iran’s national security depended on maintaining what he described as “Iranian arrangements” in the Strait of Hormuz.
In the latest attacks, the US targeted command centres, air defence sites, and coastal surveillance facilities across Iran – including in the port city of Bandar Abbas and Greater Tunb Island – US Central Command (Centcom) said.
Explosions were heard across Iran and air defences were triggered in Tehran, Iranian state media reported.
Iran’s foreign ministry spokesman Esmaeil Baqaei said a children’s cancer treatment centre in the western city of Ahvaz had been evacuated after strikes on a nearby location.
Shortly after, US allies in the Gulf reported attacks. Kuwait’s military said it intercepted drone attacks, while Bahrain’s interior ministry told citizens to remain calm and head to the nearest safe place.
The Iranian military also said it targeted US communication systems and fuel storage facilities in Jordan in its latest round of strikes.
As tensions intensify, the Strait of Hormuz – a critical waterway off Iran’s coast that Tehran effectively blocked in response to US-Israeli strikes – remains closed.
On Tuesday, the US said it had resumed a blockade on Iranian ports, which was previously lifted as part of a deal that was struck by the two countries last month – known as a memorandum of understanding.
The following day, the US fired on and disabled an unladen, Curacao-flagged oil tanker, which Centcom said was attempting to sail toward a blockaded Iranian port.
In response to the renewal of the US blockade, Iran’s Revolutionary Guard Corps warned the US that it should “expect the closure of other oil and gas export routes that serve the interests of the United States and its allies”.
It did not elaborate on which routes could be affected.
As the dispute over the Strait of Hormuz continues, there has been sharp rise in oil prices globally triggered by tanker traffic virtually stalling through the key shipping lane.
Despite the ongoing hostilities, Trump welcomed what he saw as a potential olive branch from Tehran in freeing an American detainee that the US president said had been “wrongfully detained” in December 2024.
“The United States of America appreciates this gesture of Goodwill by Iran!” Trump wrote on Wednesday on his Truth Social platform.
The woman, Dena Karari, was on her way back to the US, according to her attorney, Jared Genser, in a post on X.
